SWI/SNF Unwraps, Slides, and Rewraps the Nucleosome

Nucleosome positioning, nucleosome spacing and the nucleosome code.

Nucleosome positioning has been the subject of intense study for many years. The properties of micrococcal nuclease, the enzyme central to these studies, are discussed. The various methods used to determine nucleosome positions in vitro and in vivo are reviewed critically. These include the traditional low resolution method of indirect end-labelling, high resolution methods such as primer exten...

The Histological Slides and Drawings of Cajal

Ramón y Cajal's studies in the field of neuroscience provoked a radical change in the course of its history. For this reason he is considered as the father of modern neuroscience. Some of his original preparations are housed at the Cajal Museum (Cajal Institute, CSIC, Madrid, Spain). In this article, we catalogue and analyse more than 4,500 of Cajal's histological preparations, the same prepara...

Asymmetric Unwrapping of Nucleosomes under Tension Directed by DNA Local Flexibility

Dynamics of the nucleosome and exposure of nucleosomal DNA play key roles in many nuclear processes, but local dynamics of the nucleosome and its modulation by DNA sequence are poorly understood. Using single-molecule assays, we observed that the nucleosome can unwrap asymmetrically and directionally under force. The relative DNA flexibility of the inner quarters of nucleosomal DNA controls the...

Induction Slides

Lecture Slides

Consider the initial value problem u = f (u, t), u(t 0) = u 0 An mth order system of s equations can be reduced to a system of ms first order equations, by introducing new variables v 1 = u , v 2 = u ,. .. , v m−1 = u (m−1). The explicit time-dependency can be removed by introducing a new variable w = t with the trivial equation w = 1, to obtain a new autonomous system of equations A linear sys...